Characterization of sulfur-compound metabolism underlying wax-ester fermentation in Euglena gracilis
نویسندگان
چکیده
منابع مشابه
Lipid Metabolism of Euglena Gracilis.
In both plants and animals pathways exist for the conversion of oleic acid to polyenoic acids (1, 2), but these pathways lead to different end products in the two kingdoms. In green plants the double bond system is extended toward the methyl end of the molecule to form linoleic and linolenic acids (9,12, lBoctadecatrienoic acid) (1,3,4). The ciliated protozoa, the amoebae, and some other animal...
متن کاملCritical Involvement of Environmental Carbon Dioxide Fixation to Drive Wax Ester Fermentation in Euglena
Accumulation profiles of wax esters in Euglena gracilis Z were studied under several environmental conditions. The highest amount of total wax esters accumulated under hypoxia in the dark, and C28 (myristyl-myristate, C14:0-C14:0) was prevalent among all conditions investigated. The wax ester production was almost completely suppressed under anoxia in the light, and supplying exogenous inorgani...
متن کاملVariability of wax ester fermentation in natural and bleached Euglena gracilis Strains in response to oxygen and the elongase inhibitor flufenacet.
Euglena gracilis is able to synthesize adenosine triphosphate under anaerobic conditions through a malonyl-independent fatty acid synthesis leading to wax ester fermentation. Mitochondrial fatty acid synthesis uses acetyl-CoA and propionyl-CoA as C2- and C3-donors for de novo synthesis of even- and odd-numbered fatty acids, respectively. Euglena's wax ester fermentation has only been described ...
متن کاملMitochondrial trans-2-enoyl-CoA reductase of wax ester fermentation from Euglena gracilis defines a new family of enzymes involved in lipid synthesis.
Under anaerobiosis, Euglena gracilis mitochondria perform a malonyl-CoA independent synthesis of fatty acids leading to accumulation of wax esters, which serve as the sink for electrons stemming from glycolytic ATP synthesis and pyruvate oxidation. An important enzyme of this unusual pathway is trans-2-enoyl-CoA reductase (EC 1.3.1.44), which catalyzes reduction of enoyl-CoA to acyl-CoA. Trans-...
متن کاملPurification and characterization of pyruvate:NADP+ oxidoreductase in Euglena gracilis.
Pyruvate:NADP+ oxidoreductase was homogeneously purified from crude extract of Euglena gracilis. The Mr of the enzyme was estimated to be 309,000 by gel filtration. The enzyme migrated as a single protein band with Mr of 166,000 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, suggesting that the enzyme consists of two identical polypeptides. The absorption spectrum of the native e...
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ژورنال
عنوان ژورنال: Scientific Reports
سال: 2019
ISSN: 2045-2322
DOI: 10.1038/s41598-018-36600-z